IL-32 is induced by activation of toll-like receptors in multiple myeloma cells

Multiple myeloma (MM) is a hematological cancer characterized by accumulation of malignant plasma cells in the bone marrow. The patients are immune suppressed and suffer from recurrent and chronic infections. Interleukin-32 is a non-conventional, pro-inflammatory cytokine expressed in a subgroup of MM patients with a poor prognosis. IL-32 has also been shown to promote proliferation and survival of the cancer cells. Here we show that activation of toll-like receptors (TLRs) promotes expression of IL-32 in MM cells through NFκB activation. In patient-derived primary MM cells, IL-32 expression is positively associated with expression of TLRs. Furthermore, we found that several TLR genes are upregulated from diagnosis to relapse in individual patients, predominantly TLRs sensing bacterial components. Interestingly, upregulation of these TLRs coincides with an increase in IL-32. Taken together, these results support a role for IL-32 in microbial sensing in MM cells and suggest that infections can induce expression of this pro-tumorigenic cytokine in MM patients.


Introduction
Multiple myeloma (MM) is a hematological cancer characterized by accumulation of malignant plasma cells in the bone marrow. Clinical manifestations include high levels of monoclonal antibodies in serum and urine, anemia, multiple organ failure, immune suppression and bone disease (1). IL-32 is a pleiotropic cytokine with classical proinflammatory functions as well as more unconventional roles in cancer, autoimmune diseases, and infections (2)(3)(4). It is shown to be an important growth factor and metabolic regulator of MM cells (5). Furthermore, IL-32 is secreted by MM cells in exosomes and alters the tumor microenvironment by promoting osteoclastogenesis and bone degradation (6) as well as immune suppression (7,8). High expression of IL-32 at diagnosis is observed in 10-15% of patients and these individuals have inferior survival compared to non-expressors (5,6). Moreover, IL-32 expression is upregulated upon relapse in a fraction of patients (5).
IL-32 plays an important role in the host defense against a range of infectious agents (4) and IL-32 expression can be induced by tolllike receptor (TLR) activation in monocytes/macrophages, dendritic cells, and epithelial cells (9)(10)(11)(12)(13). TLRs recognize pathogen associated molecular patterns (PAMPs) and may also recognize and respond to danger associated molecular patterns (DAMPs) released during cell damage and cell death (14,15). MM patients suffer from chronic and recurrent fungi-, bacteria-and virus-infections (16)(17)(18). In addition, due to cancer-induced inflammation and cancer treatment there is extensive cell death in the MM bone marrow microenvironment, which may lead to the release of DAMPS. Thus, MM cells in the bone marrow are likely to be exposed to TLR agonists derived from either microbes or cellular stress.
We have previously shown that IL-32 is induced in response to hypoxia (6). Whether IL-32 expression is induced in response to TLR signaling in MM cells is not known. Thus, to determine if infections or cell death may induce cancer cell expression of IL-32 we here examined if IL-32 is induced by TLR agonists in MM cell lines and if expression of IL-32 correlates with TLR expression in primary MM cells.

Cells and culture conditions
MM cell lines ANBL-6, INA-6 and JJN-3 were kind gifts from Dr. Diane Jelinek (Mayo Clinic, Rochester, MN), Dr. Martin Gramatzki (University of Erlangen-Nuremberg, Erlangen, Germany), and Dr. Jennifer Ball (University of Birmingham, UK), respectively. RPMI-8226 and U266 were obtained from American Type Culture Collection (ATCC, Rockville, MD, USA), while IH-1 and OH-2 were established in our laboratory (19,20). RPMI-8226, U266 and JJN-3 were cultured in RPMI-1640 (RPMI) medium with 20, 15 and 10% heat inactivated fetal calf serum (FCS), respectively. ANBL-6 and INA-6 cells were cultured in RPMI with 10% FCS and the addition of 1 ng/ml recombinant human (rh) interleukin (IL)-6. OH-2 and IH-1 were cultured in RPMI containing 10% heat inactivated human serum (HS), and KJON-1 with 5% HS, both supplemented with 1 ng/mL rhIL-6. Cell lines were cultured at 37°C in a humidified atmosphere containing 5% CO 2 . RPMI-8226 TLR9 and TLR4 KO cells were generated using CRISPR/Cas9 technology followed by single cell cloning and maintained in the same culture conditions as the original RPMI cells. For LPS stimulation experiments with peripheral blood mononuclear cells (PBMCs) from healthy donors, PBMCs were isolated from fresh buffycoats by Histopaque -1077 gradient and kept in 10% RPMI medium overnight before stimulation-experiments. Freshly isolated primary myeloma cells were obtained from the local hospital biobank (Biobank1). CD138+ cells were isolated by RoboSep automated cell separator using Human CD138 Positive Selection Kit (StemCell Technologies, Grenoble, France) and later the same day, CD138+ cells were seeded in RPMI-1640 with 2% HS and 1 ng/mL rhIL-6 and stimulated with TLR ligands for 4 hours. When few primary myeloma cells were donated, the cells were stimulated with a cocktail containing all the ligands. Concentrations of ligands are described below. All patient samples were donated after informed consent, and the study was approved by the regional ethics committee (REK# 2011/2029 and REK# 247909). The study was performed in accordance with the Helsinki declaration.
TLR9 and TLR4 CRISPR/Cas9 KO cell lines RPMI-8226 cells were electroporated with TLR9 (#sc-400600) and TLR4 (sc-400068-KO-2) CRISPR/Cas9 KO and control (#sc-418922) plasmids from Santa Cruz Biotechnology, all containing green fluorescent protein (GFP) for selection. Cells were transfected using the Nucleofector ™ II Device (Lonza) with buffer V (Amaxa Nucleofector Kit V, Lonza) and program G-015. Cells were then sorted for GFP positivity on a FACSAria Fusion flow cytometer (BD Biosciences) and single cell cloned. Clones were screened for TLR expression by immunoblotting (TLR9 KO and WT) or qPCR (TLR4 KO and WT).  Figure 2F). We pre-incubated the cells with inhibitors for 30 minutes before adding LPS for further 4 hours (time-point titration as shown in Supplemental Figure 2G).

Sequencing of primary MM cells
Own dataset: CD138+ MM cells were obtained from Norwegian Myeloma Biobank (Biobank1, St. Olavs University Hospital HR, Trondheim, Norway) and isolated as previously described (6). Next, RNA was isolated from purified MM cells using miRVana total RNA isolation (ThermoFisher, #AM1560). RNA-seq was performed using the TruSeq Stranded mRNA Library Prep Kit (Illumina # RS-122-2101) according to the manufacturer's protocol using 400 ng input RNA. Illumina (#20020595, San Diego, CA, USA) followed by 75 bp single read sequencing on the Illumina Hiseq 4000 next machine. The depth of sequencing was 18 million reads per sample. The study was approved by the regional ethics committee (REK # 247909).

RNA sequencing data analyses
RNA sequencing data (MMRF_CoMMpass_IA13a_E74 GTF_Salmon_Gene_Counts) and clinical data were downloaded from the Multiple Myeloma Research Foundation CoMMpass IA13 release (https://research.themmrf.org/). RNA sequencing data from CD138 + cells were available for 795 baseline samples from patients with MM. For TLR gene expression analysis, patient samples taken at diagnosis were divided into high and low IL-32 expression based on the same percentiles (upper 10 th and lower 90 th ) as used in our previous study on IL-32 in myeloma (5). We used the upper 10th percentile (n = 54; counts per million (cpm, log2)> 1.52) and lower 90th percentile (n = 741, (cpm,log2)≤ 1.52) and significance was analyzed by one-tailed Wilcoxon signed-rank test in R. Pearson correlation analysis was performed using stat_cor function in R.
RNA-sequenced CD138 + cells from longitudinal samples were available for 47 samples in IA13. We analyzed IL-32 expression at diagnosis and first relapse timepoint and divided these into two equally large groups based on fold change (FC) upregulation of IL-32 from diagnosis to relapse. Patients with log2 FC≥2.25 (n=23) and log2 FC<2.25 (n=24) were separated into two groups. Fold change differences in TLR expression between diagnosis (baseline) and relapse were calculated for these two groups.
For own RNA sequencing data (the Biobank1 dataset), high IL-32 expressing patients were defined as those with IL-32 expression (cpm,log2) >1.57, and low/non-expressing patients were defined as those with IL-32 expression (cpm,log2) ≤ 1.57), which represents the upper 30% percentile (n=26) and lower 70% percentile (n=61), respectively, This is approximately the same cpm cutoff as used when analyzing the CoMMpass IA13 dataset. Differences in TLR gene expression were assessed using one-tailed Wilcoxon signedrank test in R.

Immunoblotting
Cells were lysed in lysis buffer (50 mM Tris-HCl, 1% NP40, 150 mM NaCl, 10% glycerol, 1 mM Na 3 VO 4 , 50 mM NaF and Complete protease inhibitor (Roche Diagnostics, Mannheim, Germany). Lysates were denatured in 1× NuPage LDS sample buffer supplemented with 0.1 mM DTT for 10 min at 70°C before they were separated on 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the iBlot Dry Blotting System (Invitrogen, Camarillo, CA, USA). Membranes were blocked using 5% bovine serum albumin (Sigma-Aldrich, St. Louis, MO) in Tris-buffered saline with 0.01% Tween followed by overnight incubation with the primary antibodies previously described. Detection was performed using horseradish peroxidase (HRP) conjugated antibodies (DAKO, Glostrup, Denmark) and developed with Super Signal West Femto Maximum Sensitivity Substrate (Thermo Scientific, Rockford, IL, USA). Images were obtained with LI-COR Odyssey Fc and analyzed using Image Studio Software (LI-COR, Lincoln, NE, USA).

Statistical analyses
Results from in vitro experiments were graphed and analyzed using GraphPad by Prism version 8 software (La Jolla, CA, USA). Patient datasets were analyzed in R, with statistical packages. Experimental replicate numbers and statistical tests are indicated in the figure legends. Briefly, mean value and standard error of the mean (SEM) were calculated for replicates from independent experiments. For replicates within one experiment mean and SD is shown. For comparison of two groups unpaired Student´s t-test was used. For comparison of two groups with measurements over time, multiple t-tests were performed. For comparison of different treatments within one group one-way ANOVA and Dunnett´s multiple comparison was used. For comparison of more than two groups with measurements over time, two-way ANOVA and Dunnett´s multiple comparisons test was used.

TLR-induced NFkB signaling promotes IL-32 expression in MM cells
We previously showed that IL-32 is expressed in a subset of MM patients and MM cell lines (6). TLRs are also expressed by primary MM cells (21,22). Both IL-32-and TLR expression varies between cell lines (Supplemental Figures 1A, B). RPMI-8226 cells have a broad repertoire of TLRs and do not express IL-32 in basal culture conditions (Supplemental Figure 1), and we therefore used these cells for TLR-stimulation. Optimal ligand concentrations used in the experiments were determined by titration ( Supplemental  Figures 2A-C). IL-32 mRNA was upregulated in RPMI-8226 in response to Pam3cys (TLR2/1), FSL-1 (TLR2/6), LPS (TLR4), R-848 (TLR7 and TLR8) and CpG (TLR9) ( Figure 1A). Moreover, IL-32 protein expression was increased in response to LPS and CpG after 4 hours, and after 24 hours, increased IL-32 protein was evident also in response to Pam3cys, FSL-1 and R-848 ( Figure 1B). Thus, increased IL-32 mRNA corresponded to increased IL-32 protein. IL-32 was transcribed after 2 hours of stimulation with LPS ( Figure 1C), while IL-32 protein was evident after 3 hours, indicating that LPS-induced IL-32 is regulated through gene transcription ( Figure 1D).
To investigate if IL-32 expression was induced by LPS and CpG through their cognate receptors, we evaluated the expression of IL-32 in RPMI-8226 cells depleted of TLR4 or TLR9 (validation of knock out in Supplemental Figures 2D, E). As expected, LPS did not promote IL-32 expression in TLR4 KO cells ( Figure 1E), while the TLR9 ligand CpG did not induce IL-32 in TLR9 KO cells ( Figure 1F), supporting that the induction of IL-32 in response to PAMPs is mediated by cognate TLR receptorligand interactions.
NFkB is a central downstream transcription factor of TLRsignaling. To investigate if transcription of IL-32 was regulated by NFkB we treated RPMI-8226 cells with two inhibitors of NFkB signaling: the IKK inhibitor VII and the TAK1/MAP4K2 inhibitor NG25. LPS-induced IL-32 was significantly reduced with NG25 and almost completely abolished by the IKK inhibitor VII, both at the mRNA level ( Figure 1G; Supplemental Figures 2F, G)) and at the protein level ( Figure 1H) indicating that NFkB is the main regulator of the LPS-induced transcription of IL-32. Expression of IL-32 following CpG stimulation was also inhibited by the IKK inhibitor, supporting that NFkB is essential for inducing IL-32 also in response to other TLR agonists (Supplemental Figure 2H). The NFkB inhibition was validated by western blotting of p-IkB where the LPS-induced increase of p-IkB was reduced upon treatment with IKK-VII (Supplemental Figure 2I). Induction of IL-32 by LPS, and inhibition of IL-32 expression by the NFkB inhibitor VII was also observed in PBMCs from healthy donors, supporting that the response may be conserved in different immune cells (Supplemental Figures 2J, K). Taken together, these results show that ligand binding and signal transduction through TLRs followed by activation of NFkB is important for the induction of IL-32 in MM cells in response to TLR agonists.

IL-32 is associated with TLR expression in primary MM cells
As we found IL-32 to be upregulated by TLR activation in vitro, we next investigated if IL-32 is induced in primary myeloma cells in response to TLR agonists (Figure 2A). Freshly obtained CD138+ primary cells from five patients were stimulated with ligands activating TLR 1-9. IL-32 was increased more than two-fold in response to TLR1/2 agonist Pam3Cys and TLR4 agonist LPS in patient #1. In patients #2 and #3 Il-32 expression was increased about 50% in response to flagellin and FSL-1, respectively (Figure 2A). For patients #4 and #5, fewer cells were donated, and we therefore used a cocktail containing all agonists for stimulation. IL-32 expression increased nearly three-fold in patient #4 following TLR agonist cocktail stimulation, while the response to TLR stimulation was less prominent in patient #5 (about 30% percent increase in IL-32 mRNA expression).
We previously showed that IL-32 is upregulated upon relapse and that some IL-32-non-expressors start to express IL-32 in the time period between diagnosis and relapse (5). To investigate if TLR expression in a similar manner is increased in samples obtained at relapse compared with samples obtained at diagnosis we analyzed the same longitudinal RNA-sequenced samples (n=47). Indeed, TLR2, TLR4, TLR5, TLR7 and TLR8 mRNA expression were significantly upregulated at treatment relapse compared with expression at time of diagnosis ( Figure 2E). Interestingly, these were the same TLRs that were most significantly associated with IL-32 in the diagnostic samples in the CoMMpass dataset ( Figure 2B) as well as in our own dataset ( Figure 2C). To investigate if the increased expression of TLRs in samples obtained at relapse coincided with the increase of IL-32, we divided the patient samples into two groups based on the fold change upregulation of IL-32 from diagnosis to relapse (logFC ≥ 2.25, n= 23 and log2FC<2.25, n=24, Supplemental Table 1) and compared changes in TLR expression between the two groups. Strikingly, patients with an IL-32 FC2.25 from diagnosis to relapse had significantly increased TLR 2-,4-,5-and 8-expression at relapse compared with expression at diagnosis. ( Figure 2F). In contrast, in the group of patients with no/small increase in IL-32 in the relapse samples, there was no significant increase in TLR expression at relapse ( Figure 2E). These data support that a subgroup of patients experience high IL-32 and high TLR expression upon disease progression.
Finally, as we found that NFkB transduced the TLR-dependent transcription of IL-32 we asked whether the fraction of patients with hyperactivated NFkB is also the IL-32 expressors. NFkB is constitutively active in 20% of MM patients and 40% of MM cell lines (24)(25)(26). We analyzed the IL-32 expression in patients from the CoMMpass IA11 dataset with and without a mutation in the NFkB pathway (27). There was however no association between IL-32 expression and mutated NFkB (Supplemental Figure 2L), indicating that hyperactivated NFkB is not the leading cause of constitutive IL-32 expression in MM patients.

Discussion
Here we demonstrate that that IL-32 is induced in MM cells in response to PAMPs through NF-kB activation downstream of TLRs. We further found that IL-32 expression is associated with TLR expression in MM patients in two large, independent patient cohorts. We also show that TLR expression in malignant plasma cells increased from diagnosis to relapse in individual patients and coincided with an increase in IL-32. IL-32 is expressed in 10-20% of patients at diagnosis, and a larger fraction of patients start to express IL-32 following treatment (5). Importantly, the expression is not linked to specific genetic subgroups (6), which supports that IL-32 expression is induced by extracellular cues. Indeed, IL-32 is upregulated in MM cells by hypoxia (6), which is a characteristic of the MM bone marrow (6,(28)(29)(30). Our in vitro and in vivo data presented here support that IL-32 expression in MM cells can also be induced by TLR-activation. TLRs are heterogeneously expressed by primary MM cells (21,22,31) and exposure of various TLR-ligands is likely to have different effects depending on the cells' TLR repertoire (32). Indeed, this is evident in our experiments with primary cells, where cells from all patients increased IL-32 mRNA in response to TLR-stimulation, but which ligands that activated the cells and to which extent IL-32 The figure shows the mean ± SEM for TLR expression at diagnosis and relapse in each group. In A, B and D, significance was analyzed by one-tailed Wilcoxon signed-rank test. In E significance was calculated by twotailed Wilcoxon signed-rank test.
was increased varied greatly. In patient sequencing data the strongest correlations were between IL-32 and TLR2 and TLR8.
In the bone marrow, TLR agonists may be derived from pathogens or from damaged or dead cells. TLR expression has been shown to increase upon infection (33)(34)(35). Thus, although this correlation is not absolute (36), high expression of TLRs in primary MM cells may be indicative of an inflammatory bone marrow microenvironment or of an ongoing infection. MM patients are susceptible to infections (16) and the anti-MM treatments increase the frequency of infection further (37). The level of DAMPs rises following anti-MM treatment due to tumor cell death. It is therefore likely that DAMPs may also be a driver of IL-32 expression in the MM bone marrow microenvironment, but this remains to be further investigated.
Intracellular IL-32 promotes proliferation and survival of MM cells (5,38) and depletion of IL-32 from myeloma cell lines reduced tumor engraftment and/or tumor growth in three different xenograft mouse models (5). At the molecular level, IL-32 interacts with components of the mitochondrial respiratory chain and promotes oxidative phosphorylation in malignant plasma cells (5). IL-32 is also expressed in plasma cells obtained from healthy individuals (5). However, whether IL-32 is induced to a similar extent upon TLR activation in non-malignant plasma cells and the role of IL-32 for normal plasma cell function needs to be elucidated.
In the cancer setting, MM cell-derived IL-32 may also have indirect effects on tumor progression. IL-32 can accelerate disease progression by promoting osteoclast differentiation and bone destruction (6.) In an early study, IL-32 was shown to promote the differentiation of monocytes into macrophages while the generation of functional dendritic cells was inhibited (39), which may suggest that IL-32 can have negative effects on immune responses. Indeed, more recently, myeloma-derived IL-32 was shown to promote the formation of immunosuppressive, M2-like macrophages (8,40,41).
In conclusion, activation of TLRs in an inflamed or infectious bone marrow microenvironment may lead to IL-32 expression, and this may contribute to accelerate the disease. We therefore propose that the subgroup of IL-32-expressing patients may benefit from combination treatments where drugs targeting hypoxia, antibiotics, antiviral-or anti-inflammatory drugs are included.

Ethics statement
The studies involving human participants were reviewed and approved by the Regional Ethics Committee 247909. The patients/ participants provided their written informed consent to participate in this study.